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ribT [2018-05-30 10:34:41]
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ribT [2018-05-30 10:34:41]

similar to GCN5-related N-acetyltransferase
locus
BSU23240
pI
5.75
mw
14.43 kDa
protein length
124 aa Sequence Blast
gene length
372 bp Sequence Blast
function
unknown
product
GCN5-related N-acetyltransferase
essential
no
synonyms
ribD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    2,427,405 → 2,427,779

    The protein

    Structure

  • [PDB|5XXS] [pubmed|29241954]

    • Expression and Regulation

    Operons

    genes
    [gene|3DFCF7C5FE15C9A3E9E7E4B0DA8E926B85DBD180|ribD]-[gene|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|ribE]-[gene|F07B7062C850106A95C15978E36DA500F8B089D6|ribA]-[gene|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|ribH]-[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]
    description
    [Pubmed|8159171]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8159171], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|FMN-box|FMN-box]: transcription termination, via [SW|FMN-box] in the presence of FMN or FMNH2, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|RibR], in [regulon|FMN-box|FMN-box]

    • regulation

  • expressed in the absence of FMN ([SW|FMN-box]) [Pubmed|15808508]
  • binding of FMN to the [SW|FMN-box] RNA is facilitated by [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|NusG]-mediated transcription pausing [pubmed|32817529]
  • the [SW|FMN-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    Biological materials

    Mutant

  • BKE23240 (Δ[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACCCCTCTATATC, downstream forward: _UP4_TAAGCAGAGGCTGTGATCAG
  • BKK23240 (Δ[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACCCCTCTATATC, downstream forward: _UP4_TAAGCAGAGGCTGTGATCAG

    • References

  • 12456892,15808508,23270261,8159171,7934830,24442413,25349719,26494285,29241954